The article was published on Biosensors and Bioelectronics in July 2019. The authors Zhipeng Huang et al (Department of Chemistry, Fudan University, Shanghai, China) developed a rapid, sensitive and inexpensive method for albumin detection based on the polydopamine (PDA) bi-functionalized glass chip and surface-enhanced Raman scattering (SERS) tag.
Background & Aims & Conclusions
Albumin is a widely used clinical indicator in patients with diabetes, kidney and cardiovascular disease (CV). The current detection method for albumin has disadvantages such as long detection time and high detection limit.
The authors aim to develop a rapid, sensitive and environmentally friendly albumin detection method.
Albumin antibody was encapsulated on the glass in the process of dopamine self-polymerization. The microalbuminuria was captured and enriched onto the chips by the antibody exposed on the surface of the glass. Then the PDA chips were incubated with antibody modified 4-Mercaptopyridine (4-MPY) SERS tag forming a chip-albumin-SERS tag sandwich structure. The detection of proteins such as hemoglobin (Hgb) and human immunoglobulin G (IgG) indicates that the SERS method has high specificity and selectivity.
Part II: The Brief Introduction of Our Product
In this experiment, anti-albumin antibodies was purchased from CUSABIO.
Immunofluorescent analysis of HepG2 cells using CSB-PA001561LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
