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Á¦¸ñ [Cusabio] New Product Launch! E-Tag Monoclonal Antibody, Available!
ÀÛ¼ºÀÚ abbabio
ÀÛ¼ºÀÏÀÚ 2019-09-26
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E-TAG (amino acid sequence: GAPVPYPDPLEPR) is a common epitope tag with 13 amino acids and a molecular weight of approximately 1.4 kDa. E-TAG tag antibody can specifically recognize the fusion protein with E-TAG tag, which can effectively detect the expression of E-TAG fusion-expressed protein.

CUSABIO is now launching E-TAG tag antibodies, and validated applications include ELISA, WB and IP.

Product Card

  • Product Name:E-Tag Monoclonal Antibody
  • Code: CSB-MA000151M0m
  • Raised in: Mouse
  • Species Reactivity: All
  • Tested Applications: ELISA, WB, IP
  • More Detail >>

The Show of Product

  • ELISA
  • E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
  • Antigen at dilution of 1µg/ml
  • E-Tag antibody at (0-0.15µg)
  • Goat polyclonal to Mouse IgG at 1/50000 dilution
  • Recommended dilution ratio: 1:4000-1:8000
  • Protocol
  • Western Blot (WB)
  • Positive WB detected in: E-tagged fusion protein
  • All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
  • Predicted band size: 36 kDa
  • Observed band size: 36 kDa
  • Recommended dilution ratio: 1:1000-1:320000
  • Protocol
  • Immunoprecipitation (IP)
  • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
  • Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
  • Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
  • Lane 3: 293F transfected whole cell lysate (20µg)
  • Recommended sampling amount: 1µg-5µg
  • Protocol
Kit
Protein
Antibody
Molecular Biology
CUSABIO TECHNOLOGY LLC
Postal Address: 7707 Fannin St.£¬Ste 200-V126£¬Houston TX 77054
Tel: 301-363-4651£¨Available 9 a.m. to 5 p.m. CST from Monday to Friday£©
Website: www.cusabio.com
© 2019 CUSABIO All Products For Research Use Only
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