Adeno-associated virus (AAV) has been the platform of choice for in vivo gene therapy in recent years. This is not only due to AAV's high efficiency in transducing genes into target tissues, but also its superior safety profile. However, a recent study in dogs with hemophilia showed that AAV can readily insert its payload into host DNA and induce cancer. >>> Read More
Read this case study to learn the advantages of using long single-stranded DNA (ssDNA or ssODN) as non-viral CRISPR HDR templates in cell engineering.
√ Precise gene knock-in, non-viral, no random insertion √ High gene knock-in efficiency √ Reduced off-target integration √ Low cell toxicity
GenScript offers high quality, sequence verified long single-stranded DNA (ssDNA or ssODN) for maximizing the editing efficiency and accuracy of your CRISPR homology directed repair (HDR) based gene knock-in experiments.
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151-3000 nt in length;
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Sequence verified by sanger sequencing the final ssDNA product;
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Up to 20 ug delivery quantity allowing for flexible study design;
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Enzymatic approach for ensuring non-detectable levels of dsDNA.
