Accumulating evidences have showed mutations in structural proteins of virus play crucial role in viral virulence by determining generation of antibody escape variants and cellular tropism. A recent study of Arup Kumar Banerjee et al. from India have pointed out D614G is clearly a very frequent mutation, and this type of mutation doesn’t fall in the RBD of spike protein. This D614G mutation has rapidly become dominant in these regions, suggesting this mutation may make the SARS-CoV-2 virus more transmissive. It's critical to understand the biology of this variant, particularly in the context of vaccine and drug design.

Now, CUSABIO have developed a recombinant S1 protein with a D614G mutation (CSB-MP3324GMY(M1)). This mutant protein reagent have demonstrated an ACE2 binding activity by functional ELISA. In addition to the D614G mutation, several other mutations are also identified, including RBD (V367F), RBD (W436R), RBD (G476S) and RBD (V483A).

Partial Product Verification Chart

Recombinant Human Novel Coronavirus Spike glycoprotein(S) (D614G), partial (CSB-MP3324GMY(M1))
	Purity: Greater than 90% as determined by SDS-PAGE (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.		Functional ELISA. Immobilized SARS-CoV-2-S1 (D614G) at 2 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 8.236-11.22 ng/ml.
Recombinant Human Novel Coronavirus Spike glycoprotein(S) (V367F), partial (CSB-MP3324GMY1(M1))
	Purity: Greater than 90% as determined by SDS-PAGE (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.		Functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V367F) at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 65.58-90.16 ng/ml.
Recombinant Human Novel Coronavirus Spike glycoprotein(S) (G476S), partial (CSB-MP3324GMY1(M4))
	Purity: Greater than 90% as determined by SDS-PAGE (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.		Functional ELISA. Immobilized SARS-CoV-2-S1-RBD (G476S) at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 47.11-92.3 ng/ml.

All of Spike Mutant Proteins of SARS-CoV-2

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