(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody (CSB-RA33245D1GMY), the EC50 is 106.2-131.2 ng/ml.
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml.
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized L1CAM at 2 μg/ml can bind Anti-L1CAM Rabbit Monoclonal Antibody （CSB-RA595071A0HU）, the EC50 is 5.384-9.380 ng/ml.
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This assay employs the competitive inhibition ELISA for the qualitative determination of SARS-CoV-2 neutralizing antibody concentrations in serum, plasma. The standard curve is provided for demonstration only. It should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
ng/ml
OD1
OD2
Average
10000
0.099
0.092
0.096
156
0.901
0.907
0.904
5000
0.145
0.136
0.141
78
1.509
1.412
1.461
2500
0.194
0.183
0.189
39
1.787
1.723
1.755
1250
0.274
0.263
0.269
19.5
2.121
1.958
2.040
625
0.344
0.371
0.358
9.75
2.233
2.138
2.186
312
0.519
0.528
0.524
0
2.394
2.328
2.361
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