(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 ¥ìg/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
This assay employs the Sandwich ELISA for the quantitative determination of SARS-CoV-2 S1 RBD concentrations in serum, plasma, swabs. The standard curve is provided for demonstration only. It should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
80
2.648
2.594
2.621
2.409
40
1.973
2.032
2.003
1.791
20
1.027
1.069
1.048
0.836
10
0.559
0.537
0.548
0.336
5
0.365
0.358
0.362
0.150
2.5
0.291
0.285
0.288
0.076
1.25
0.251
0.240
0.246
0.034
0
0.213
0.211
0.212
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