(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 μg/ml can bind TNFSF18 (CSB-MP891791HU), the EC50 is 2.565 to 2.940 ng/ml. recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Human TNFRSF18 protein hFc tag (CSB-MP896537HU) captured on COOH chip can bind Human TNFSF18 protein hFc and Flag tag with an affinity constant of 38.5 nM as detected by LSPR Assay.
This assay employs the indirect ELISA for the qualitative determination of SARS-CoV-2 N/S1 IgG Antibody concentrations in serum, plasma.
Precision
Intra-assay Precision (Precision within an assay): CV% < 15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% < 15%
Three samples of known concentration were tested in twenty assays to assess.
Typical Data
Test parameter
Specification
Test result
Positive control
≥ 0.8
1.174
Negative control
≤ 0.25
0.187
Positive rate
20, Positive
100%
Negative rate
20, Negative
100%
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