Cusabio) NEFL Provides Insights into Neuropathologies
작성자
abbabio
작성일자
2021-07-21
조회수
352
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Neurofilament light polypeptide (NEFL) protein is a marker of neuroaxonal damage. Abnormal __expression__ of NEFL is associated with a variety of diseases including amyotrophic lateral sclerosis, multiple sclerosis, Alzheimer's disease, Charcot-Marie Tooth disease, and more recently Huntington's disease. Some studies have reported aberrant __expression__ of NEFL in neurofibromas, head and neck tumors, breast cancer, cervical squamous carcinoma, and lung cancer. Since further investigation must wait until the researchers have gathered more data, there is a large unmet need for NEFL reagents.
CUSABIO has committed to providing the best products and service to enable researchers to better conduct research. At present, we launched the recombinant NEFL proteins (CSB-EP015688HU; CSB-YP015688MO). Particularly, the recombinant human NEFL protein is available right now, which might accelerate the NEFL based studies for our scientists.
Product Information
Purity>90%
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Purity>90%
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Q: Concerning the Recombinant Human Neurofilament light polypeptide, I have a question about the tag(s) and the possibility to obtain the protein with a free N-terminus (if possible also C-terminal free). From the SUMO-tag, there exists the possibility to remove by protease at the end of the purification process.
A: The protein of CSB-EP015688HU that we currently developed is N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged.
If you need to remove the tag or tag-free protein, please communicate with us in advance. For the C-terminal tag, we can definitely guarantee to remove, however, for the N-terminal tag, we can try enzyme digestion, but we can't guarantee 100% successfully. Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge an extra cost.
If we fail in removing the tag, we won't charge for any extra cost and remark this information in the datasheet as follows "Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact."
Generally, the delivery time will be extended for 3 days.
Q: We order this protein CSB-EP015688HU several times and are satisfied with the test result, is there any discount for bulk orders?
A: Yes, we can offer an additional discount for bulk orders, please let us know your demanded quantity and your requirement for the package and shipping format.
Q: Does CSB-EP015688HU contain imidazol?
A: We have performed dialysis to change the buffer, and the imidazole is removed, but the final situation is not tested, so there is no guarantee that there will be no imidazole at all.
As the COVID-19 pandemic evolves, we are with you during these turbulent times. CUSABIO appreciates the critical role that you and our products play in research efforts to further scientific innovation and discovery. We know that many scientists are diligently pursuing their research throughout the pandemic. Our customer service and technical support teams are equipped to remain available to help with your research needs and answer any questions.
