[CD Bioparticles] Magnetic Particles for Next-generation Sequencing (NGS)
작성자
abbabio
작성일자
2021-12-22
조회수
297
Magnetic Particles for Next-generation Sequencing (NGS)
Magnetic particles are essential components in the process of next-generation sequencing (NGS). The target fragments in the sample can be separated according to the principle that active groups of magnetic particles can bind and dissociate with nucleic acids under certain conditions. Magnetic particles have achieved the high-throughput automatic operation of nucleic acid samples and are widely used for gene sequencing and molecular diagnostics.
Commercial magnetic particles product systems generally include magnetic particles, polyethylene glycol (PEG), and salt ions. Based on SPRI (Solid Phase Reversible Immobilization) technology, during the presence of PEG, NaCl, or MgCl2, DNA conformation changes sharply and exposes negatively charged phosphate groups on the phosphoric acid skeleton and binds to negatively charged carboxyl magnetic particles. The negatively charged phosphate group forms an ion bridge between the dissociated salt ions (such as Na+) and the carboxyl group so that DNA is adsorbed explicitly on the surface of carboxyl magnetic particles. Applying the magnetism of magnetic particles, they can be recycled and eluted by an external magnetic field, and DNA fragments of different lengths can be selectively precipitated.
CD Bioparticles provides a wide range of high-quality magnetic particles for nucleic acid extraction, NGS library preparation and target enrichment.
