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[Kerafst] DNA-Binding Fluorescent Probes for Increased Brightness and Two-Channel Imaging

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abbabio

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2022-01-12

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1692

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DNA-Binding Fluorescent ProbesFrom the laboratory of James N. Wilson, PhD, University of Miami
Low cytotoxicity, membrane permeable, and live-cell compatible fluorescent probes offering increased brightness and two-channel imaging
Learn more >>

Available Probes:

Miami Green (MG) and Miami Yellow (MY): nuclear dyes shown to be brighter than traditional stains such as DAPI or Hoechst
Miami Orange (MO) and Miami Red (MR): allow for two-channel imaging of nuclear and cytosolic compartments via one probe

Probe References:

Pitter DR, et al. One probe, two-channel imaging of nuclear and cytosolic compartments with orange and red emissive dyes. Org Biomol Chem. 2015 Sep 28;13(36):9477-84.
Wilson JN, et al. Base pair sensitivity and enhanced ON/OFF ratios of DNA-binding: donor-acceptor-donor fluorophores. J Phys Chem B. 2013 Oct 10;117(40):12000-6.
Pitter DR, et al. Turn-on, fluorescent nuclear stains with live cell compatibility. Org Lett. 2013 Mar 15;15(6):1330-3.
Quyen TL, et al. Classification of multiple DNA dyes based on inhibition effects on real-time loop-mediated isothermal amplification (LAMP): prospect for point of care setting. Front Microbiol. 2019 Oct 15;10:2234.

Wondering which probe is best for your research?
Check out the below excitation and emission spectra of each of the four dyes bound to DNA.

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