Cellular autofluorescence is a common problem for all fluorescence-based cell detection methods. The cells’ natural fluorescence is caused by the presence of various biological structures such as collagen, elastin, NADPH, etc., which interferes with analysis by reducing signal sensitivity and resolution of fluorochromes like FITC, GFP, and Pacific Blue. To subtract this background and prevent false positives, it is crucial to include unstained controls and set proper PMTs and gating. However, it is even more important to minimize autofluorescence as much as possible. Click “Find out how” to learn 5 methods that help reduce autofluorescence.
Boster takes great measures to ensure product quality and to provide our customers with comprehensive data upfront. Our antibodies are validated using WB, IHC, and flow cytometry against a panel of over 250 tissues and un-transfected cell lines to ensure high affinity and crystal-clear IHC stains. In addition, we also validate our antibodies in a quantitative fashion by testing them on known quantities of recombinant proteins so that you know what to expect (e.g. if there is 1ng, 2.5ng or 5ng of the target protein in the sample). Every product is covered by the Boster Quality Guarantee, click below to learn more.
