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Á¦¸ñ [Cusabio] CUSABIO Star Product-lacZ Monoclonal Antibody
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ÀÛ¼ºÀÏÀÚ 2019-06-13
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lacZ Monoclonal Antibody from CUSABIO

LacZ, also known as Beta-galactosidase, ¥â-galactosidase, or Lactase, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) into monosaccharides and encoded by the gene lacZ.

LacZ is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the lacZ gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low.

CUSABIO provides a series of lacZ antibodies with wide validated application. Here, we show one product which is the most popular with customers among these antibodies.

Product Card:

  • Product Name: lacZ Monoclonal Antibody
  • Code: CSB-MA009476A0m
  • Raised in: Mouse
  • Species Reactivity: Escherichia coli
  • Tested Applications: ELISA, WB, IF, IP, FC

Applications Show:

  • Western Blot
    Positive WB detected in Recombinant protein
  • All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
  • Predicted band size: 117 kDa
  • Observed band size: 130 kDa
  • Western Blot
    Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
  • All lanes: lacZ antibody at 1:2000
  • Predicted band size: 117 kDa
  • Observed band size: 130 kDa
  • Immunofluorescence
    Immunofluorescence staining of 293 transfected cells with CSB-MA009476A0m at 1:100, counter-stained with DAPI.
  • Immunoprecipitation
    Immunoprecipitating lacZ in 293F transfected whole cell lysate
  • Lane 1: Mouse control IgG (1µg) instead of CSB-MA009476A0m in 293F transfected whole cell lysate.
  • Lane 2: CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
  • Lane 3: 293F transfected whole cell lysate (20µg)
  • Flow Cytometry
    Overlay histogram showing 293 transfected cells stained with CSB-MA009476A0m (red line) at 1:200.
  • Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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CUSABIO TECHNOLOGY LLC
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Tel: 301-363-4651£¨Available 9 a.m. to 5 p.m. CST from Monday to Friday£©
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