[antibodies-online] Methylome uncovered - tools for MeDIP, hMeDIP, TAB-seq, and bisulfite sequencin
작성자
abbabio
작성일자
2019-06-20
조회수
764
Dear Sir or Madam,
Modification at the 5-carbon of cytosine in DNA is an epigenetic mark that influences gene expression, cell differentiation and embryonic development. While 5-methylcytosine (5-mC) is the most common and widely studied DNA-methylation, cytosine methylation and demethylation processes give rise to various other forms. In particular 5-hydroxymethylcytosine (5-hmC) has garnered interest in recent years.
5-hmC has been found to be elevated in certain mammalian tissues, such as human and mouse brain, as well as in embryonic stem cells. It is thought to determine developmental fates and, when present in aberrant patterns, to contribute to disease. A complete understanding of the potential function 5-hmC requires an accurate, scalable sequencing method.
Traditional bisulfite sequencing or methylated CpG island recovery assay (MIRA) cannot distinguish between 5-methylcytosine (5-mC), the most common and widely studied DNA-methylation, and 5-hmC.
TAB-seq (Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine) is a modification of the standard bisulfite sequencing method through which 5-hydroxymethylcytosine (5-hmC) can be specifically identified. In conjunction with the traditional methods TAB-seq allows mapping of both methylation variants at single-base resolution either on a genomic scale or for a particular locus.
genomics-online now offers TAB-seq kits and DNA standards for TABseq. Please check out our catalog or get in touch with us for more information.
Best regards,
Stefan Pellenz, Ph.D.
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